LymphedemaV1, Main, Exploration, bibRecord, 001C93

Identification of embryonic lethal genes in humans by autozygosity mapping and exome sequencing in consanguineous families

Identifieur interne : 001C93 ( Main/Exploration ); précédent : 001C92; suivant : 001C94

Identification of embryonic lethal genes in humans by autozygosity mapping and exome sequencing in consanguineous families

Source :

Genome Biology [ 1465-6906 ] ; 2015.

RBID : PMC:4491988

Abstract

Background

Identifying genetic variants that lead to discernible phenotypes is the core of Mendelian genetics. An approach that considers embryonic lethality as a bona fide Mendelian phenotype has the potential to reveal novel genetic causes, which will further our understanding of early human development at a molecular level. Consanguineous families in which embryonic lethality segregates as a recessive Mendelian phenotype offer a unique opportunity for high throughput novel gene discovery as has been established for other recessive postnatal phenotypes.

Results

We have studied 24 eligible families using autozygosity mapping and whole-exome sequencing. In addition to revealing mutations in genes previously linked to embryonic lethality in severe cases, our approach revealed seven novel candidate genes (THSD1, PIGC, UBN1, MYOM1, DNAH14, GALNT14, and FZD6). A founder mutation in one of these genes, THSD1, which has been linked to vascular permeability, accounted for embryonic lethality in three of the study families. Unlike the other six candidate genes, we were able to identify a second mutation in THSD1 in a family with a less severe phenotype consisting of hydrops fetalis and persistent postnatal edema, which provides further support for the proposed link between this gene and embryonic lethality.

Conclusions

Our study represents an important step towards the systematic analysis of “embryonic lethal genes” in humans.

Electronic supplementary material

The online version of this article (doi:10.1186/s13059-015-0681-6) contains supplementary material, which is available to authorized users.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491988

DOI: 10.1186/s13059-015-0681-6
PubMed: 26036949
PubMed Central: 4491988

Affiliations:

Arabie saoudite, Égypte

Le document en format XML

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<author><name sortKey="Ibrahim, Niema" sort="Ibrahim, Niema" uniqKey="Ibrahim N" first="Niema" last="Ibrahim">Niema Ibrahim</name>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Identifying genetic variants that lead to discernible phenotypes is the core of Mendelian genetics. An approach that considers embryonic lethality as a bona fide Mendelian phenotype has the potential to reveal novel genetic causes, which will further our understanding of early human development at a molecular level. Consanguineous families in which embryonic lethality segregates as a recessive Mendelian phenotype offer a unique opportunity for high throughput novel gene discovery as has been established for other recessive postnatal phenotypes.</p>
</sec>
<sec><title>Results</title>
<p>We have studied 24 eligible families using autozygosity mapping and whole-exome sequencing. In addition to revealing mutations in genes previously linked to embryonic lethality in severe cases, our approach revealed seven novel candidate genes (<italic>THSD1</italic>
, <italic>PIGC</italic>
, <italic>UBN1</italic>
, <italic>MYOM1</italic>
, <italic>DNAH14</italic>
, <italic>GALNT14</italic>
, and <italic>FZD6</italic>
). A founder mutation in one of these genes, <italic>THSD1</italic>
, which has been linked to vascular permeability, accounted for embryonic lethality in three of the study families. Unlike the other six candidate genes, we were able to identify a second mutation in <italic>THSD1</italic>
 in a family with a less severe phenotype consisting of hydrops fetalis and persistent postnatal edema, which provides further support for the proposed link between this gene and embryonic lethality.</p>
</sec>
<sec><title>Conclusions</title>
<p>Our study represents an important step towards the systematic analysis of “embryonic lethal genes” in humans.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s13059-015-0681-6) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
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</TEI>
<affiliations><list><country><li>Arabie saoudite</li>
<li>Égypte</li>
</country>
</list>
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<name sortKey="Rahbeeni, Zuhair" sort="Rahbeeni, Zuhair" uniqKey="Rahbeeni Z" first="Zuhair" last="Rahbeeni">Zuhair Rahbeeni</name>
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</country>
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</record>

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Identification of embryonic lethal genes in humans by autozygosity mapping and exome sequencing in consanguineous families

Identification of embryonic lethal genes in humans by autozygosity mapping and exome sequencing in consanguineous families

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